Quantification of cannabinoids in cannabis plant material and cannabis based products is performed by High Pressure Liquid Chromatography (HPLC). The choice of HPLC is related to the superiority of HPLC as an analytical tool for determination of components in herbal medicines. As opposed to GC, the main alternative analytical method, HPLC directly detect acidic compounds, allowing their accurate quantification. This hold special significance in cannabis as the natural predominant form of cannabinoids is the acidic parental compound. Therefore, HPLC enables characterization and quantification of cannabinoids in their natural form. Furthermore, our method validation is performed under USP guidelines.

Analyzed Samples:

  1. Plant material
  2. Cannabis oil extracts
  3. Cannabis oil products

Analysis includes:

  1. Extraction of the cannabinoids and preparation of sample for measurement in the HPLC instrument.
  2. Quantification of cannabinoids: THC, THCA, CBD, CBDA, CBN, CBG, CBGA, THCV and CBC.
  3. Loss on Drying.

Understanding your Results:

  1. The results are provided as the weight percentage of the individual cannabinoid in the sample.
  2. The results reflect the percentage of the cannabinoids in the naive sample, no prior treatment of the sample is conducted.
  3. Total equivalent cannabinoid provides the potential percentage of active cannabinoid (neutral form) if complete decarboxylation occur.
  4. Loss on Drying provides the percent of water in the sample and is used to calculate total equivalent cannabinoid in dry sample.